Kits
S100A12 (IDK®S100A12), ELISA
Kits
Description
Alternative names: Calgranulin C, EN-RAGE
Members of the S100 protein family are low molecular mass acidic proteins characterised
by cell-type-specific expression and the presence of 2 EF-hand calcium-binding
domains. The S100 proteins or calgranulins are expressed in activated granulocytes
under conditions of chronic inflammation. Hofmann et al. (1999) reported that RAGE
is a central cell surface receptor for S100A12, which they referred to as EN-RAGE (Extracellular
Newly identified RAGE-binding protein), and related members of the S100/
calgranulin super family. Interaction of S100A12 with cellular RAGE on endothelium,
mononuclear phagocytes, and lymphocytes triggered cellular activation, with generation
of key pro-inflammatory mediators. In murine models, blockade of S100A12
quenched delayed-type hypersensitivity and inflammatory colitis by arresting activation
of central signaling pathways and expression of inflammatory mediators.
Kaiser et al. (2007) determined the S100A12 concentration in stool samples of 171
patients suffering from infectious gastroenteritis, Crohn’s disease, ulcerative colitis
or irritable bowel syndrome and 24 healthy controls. Faecal S100A12 correlated with
the degree of intestinal inflammation. Patients with inflammatory bowel disease had
significantly higher S100A12 levels compared to these with irritable bowel syndrome
or healthy controls. The S100A12 content in stool correlated with the degree of intestinal
inflammation.
Faecal S100A12 is a novel non-invasive marker distinguishing inflammatory bowel
disease (IBD) from irritable bowel syndrome (IBS) or healthy individuals with a high
sensitivity and specificity. S100A12 stool concentrations can be used as an indicator
of inflammation activity in Morbus Crohn‘s disease and Colitis ulcerosa. Since
S100A12 is secreted by activated neutrophils and acts as a pro-inflammatory mediator,
it can be used as marker for diagnosis and therapeutic control of inflammatory
bowel diseases.
Members of the S100 protein family are low molecular mass acidic proteins characterised
by cell-type-specific expression and the presence of 2 EF-hand calcium-binding
domains. The S100 proteins or calgranulins are expressed in activated granulocytes
under conditions of chronic inflammation. Hofmann et al. (1999) reported that RAGE
is a central cell surface receptor for S100A12, which they referred to as EN-RAGE (Extracellular
Newly identified RAGE-binding protein), and related members of the S100/
calgranulin super family. Interaction of S100A12 with cellular RAGE on endothelium,
mononuclear phagocytes, and lymphocytes triggered cellular activation, with generation
of key pro-inflammatory mediators. In murine models, blockade of S100A12
quenched delayed-type hypersensitivity and inflammatory colitis by arresting activation
of central signaling pathways and expression of inflammatory mediators.
Kaiser et al. (2007) determined the S100A12 concentration in stool samples of 171
patients suffering from infectious gastroenteritis, Crohn’s disease, ulcerative colitis
or irritable bowel syndrome and 24 healthy controls. Faecal S100A12 correlated with
the degree of intestinal inflammation. Patients with inflammatory bowel disease had
significantly higher S100A12 levels compared to these with irritable bowel syndrome
or healthy controls. The S100A12 content in stool correlated with the degree of intestinal
inflammation.
Faecal S100A12 is a novel non-invasive marker distinguishing inflammatory bowel
disease (IBD) from irritable bowel syndrome (IBS) or healthy individuals with a high
sensitivity and specificity. S100A12 stool concentrations can be used as an indicator
of inflammation activity in Morbus Crohn‘s disease and Colitis ulcerosa. Since
S100A12 is secreted by activated neutrophils and acts as a pro-inflammatory mediator,
it can be used as marker for diagnosis and therapeutic control of inflammatory
bowel diseases.