S-Adenosylmethionine (SAM) ELISA Kit

Alternate Name :

Tag Line :

A competitive ELISA kit for the quantitative measurement of S-Adenosylmethionine in serum, plasma, culture supernatants and other biological fluids.

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of SAM in serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of SAM in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.39 – 25 μg/ml
Sensitivity: < 0.234 μg/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
No significant cross-reactivity or interference with its analogues was observed.

Description:

S-Adenosylmethionine (SAM) is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur throughout the body, most SAM-e is produced and consumed in the liver. In bacteria, SAM-e is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis. In eukaryotic cells, SAM-e serves as a regulator of a variety of processes including DNA, tRNA, and rRNA methylation; immune response; amino acid metabolism; transsulfuration; and more. In plants, SAM-e is crucial to the biosynthesis of ethylene, an important plant hormone and signaling molecule. BioVision’s SAM ELISA kit is a sandwich ELISA assay for the quantitative measurement of SAM in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of SAM captured from the samples.