RTEL1 (Rat) ELISA Kit

Kits

貨號 Cat. No.
E4812-100
規格 Size
E4812-100
Reactivity
Rat
Sample Type
Other biological fluids 、 Plasma 、 Serum 、 Tissue lysate

Description




Alternate Name : Regulator of telomere elongation helicase 1


 

Tag Line :


A Sandwich ELISA kit for the quantitative measurement of RTEL1 in Rat plasma, tissue homogenates and other biological fluids.


 

Storage Conditions: 4°C


 

Shelf life: 6 months


 

Summary :



 

Detection Method: Sandwich ELISA, Absorbance (450 nm)


 

Features & Benefits:


Sensitivity: 46.875 pg/ml,Detection Range: 78.125-5000 pg/ml


 

Description:


Regulator of telomere elongation helicase 1 is an ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. It acts as an anti-recombinase to counteract toxic recombination and limits crossover during meiosis. A 96-well plate was coated with a capture antibody. BioVision’s RTEL1 (Rat) ELISA Kit is based on the Sandwich ELISA principle. A 96-well ELISA microplate was coated with capture antibody the biotin-conjugated antibody was used as a detection antibody. The standards, test samples, and biotin conjugated detection antibody were added to the wells subsequently and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding the acidic stop solution. The density of color is proportional to the amount of RTEL1 captured from the samples.


 





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