rStichodactyla Toxin (ShK)
- 貨號 Cat. No.
- RTS-400
- 規格 Size
- 5 μg 5x5 μg 10μg 10μgx10μg
Description
rStichodactyla Toxin (ShK)
Kv Channel Blocker
| Product#: | RTS-400 |
| Sizes: |
| 5 µg |
| 5 x 5 µg |
| 10 µg |
| 5 x 10 µg |
| M.W.: | 4055 Da |
| Sequence: | RSCIDTIPKS RCTAFQCKHS MKYRLSFCRK TCGTC |
| Purity: | >99% HPLC |
| Bioassay: |
Irreversible inhibition of Kv1.3 channels by rStichodactyla toxin. |
 |
KV1.3 channel current expressed in Xenopus (in 2mM K+), elicited by 100 ms depolarization from holding potential of ?00 mV to 0 mV, before and during application of rStichodactyla toxin (#RTS-400) at the indicated concentrations. oocytes |
| References: |
1. | Castaneda, O. et al. (1995) Toxicon 33, 603. |
2. | Pennington, M. W. et al. (1995) Int. J. Pept. Protein Res. 46, 354. |
3. | Beeton, C. et al. (2003) J. Biol. Chem. 278, 9928. |
4. | Lizhen, Yan. et al. (2005) Mol. Pharmacol. 67, 1513. |
5. | Middleton, R.E. et al. (2003) Biochemistry 42, 13698. |
6. | Yan, L. et al. (2005) Mol. Pharmacol. 67, 1513. |
ShK blocks Kv1.3, Kv1.1, Kv1.4, and Kv1.6 at subnanomolar concentrations and Kv3.2 channels at 1000-fold higher concentration than thatrequired to inhibit Kv1.3 channels. It has been shown to block Kv current in DRG neurons, to displace radioactive Dendrotoxin from brain synaptosomes1 and inhibit I125-Charybdotoxin binding to Jurkat T lymphocytes with an IC50 of 32 pM. 2,4
Evidence also suggests thatnative Kv currents in the central nervous system, which arepredominantly carried by Kv1.2 channels, are highly sensitiveto this toxin.5
A fluorescently labeled synthetic ShK is used to recognize and to sort Kv1.3 containing lymphocytes.4,6
Alomone Labs is pleased to offer rStichodactyla (rShK), a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.