rSNX-482
- 貨號 Cat. No.
- RTS-500
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rSNX-482
R-Type Ca2+ (Cav2.3) Channel Blocker
| Product#: | RTS-500 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 4495 |
| Purity: | >98% |
| Bioassay: |
| Recombinant SNX-482 inhibits Cav2.3 currents in Xenopus oocytes |
 |
Cav2.3 channel subunits a1E, b2a and a2d were exogenously co-expressed in Xenopus Laevis oocytes. Using TEVC, membrane potential was held at -100 mV. Ba2+ (10mM) currents via Cav2.3 channels were elicited by 40 ms long voltage ramps from -100 to +60 mV, delivered every 10 seconds. Left: Representative current traces before and following the application of 100, 200 and 400 nM rSNX-482 (#RTS-500) (as indicated). Right: Dose response for rSNX-482 (n=2-6). |
SNX-482 is a peptidyl toxin originally isolated from venom of the spider Hysterocrates gigas. Native SNX-482 blocks specifically Cav2.3 (a1E, R-type) channels1 in a voltage dependent manner. The block is reversible only upon application of strong voltage to facilitate unbinding.2 SNX-482 inhibits human Cav2.3 channels stably expressed in a mammalian cell line. An IC50 of 15-30 nM was obtained for block of Cav2.3 channel, using either patch clamp electrophysiology or K+-evoked Ca2+ flux. At low nanomolar concentrations, SNX-482 also blocked a native R-type Ca2+ current in rat neurohypophyseal nerve terminals, but concentrations of 200-500 nM had no effect on R-type Ca2+ currents in several types of rat central neurons.1 SNX-482 was also used to demonstrate the contribution of Cav2.3 channels to transmitter release.3 Recently it was shown that higher concentrations of SNX-482 also block Cav2.1 channels in chromaffin cells.4
Alomone Labs is pleased to offer rSNX-482, a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.