rSlotoxin
- 貨號 Cat. No.
- RTS-410
- 規格 Size
- 5 μg 5x5 μg 10μg 10μgx10μg 0.1mg
Description
rSlotoxin
A Novel, High Conductance Ca2+-Activated K+ (BK, KCa1.1) Channel Blocker
| Product#: | RTS-410 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 4086 |
| Sequence: | TFIDV DCTVS KECWA PCKAA FGVDR GKCMG KKCKC YV |
| Purity: | >98% |
| Bioassay: |
| The biological activity of rSlotoxin was tested using two-electrode voltage clamp recording from Xenopus oocytes expressing mSlo (a1 subunit alone) channels. 100 nM slotoxin completely abolished the mSlo currents, in a reversible manner. |
 |
| The effect of 100 nM rSlotoxin on heterologously expressed BK currents (mSlo, RNA injected to Xenopus oocytes). Left: Time course of current amplitude changes upon application of the toxin (bars represent the time of toxin perfusion). Right: An example of current response to 100 ms depolarization to +20 mV (from holding potential of ?00 mV) before (red) and during (black) perfusion of 100 nM toxin. |
| References: |
1. | Garcia-Valdes, J. et al. (1998) FEBS. Lett. 505, 369. |
Native Slotoxin was originally isolated from Centruroides noxius scorpion venom.
Native Slotoxin blocks the Maxi K+ (BKCa or slo, KCNMA1, KCa1.1) channels.1
Slotoxin blocks differentially channels formed by the a1 subunit alone and channels
formed by the a1 combined with auxiliary b subunits. In Xenopus oocytes expressing
hSlo (a1 alone), Kd was calculated to be 1.5 nM and the block is complete and reversible.
With the additional co-expression of either the b1 or b4 subunits, the block become
irreversible or causes the channel to be almost insensitive to the toxin, respectively.1
Native Slotoxin blocks the Maxi K+ (BKCa or slo, KCNMA1, KCa1.1) channels.1
Slotoxin blocks differentially channels formed by the a1 subunit alone and channels
formed by the a1 combined with auxiliary b subunits. In Xenopus oocytes expressing
hSlo (a1 alone), Kd was calculated to be 1.5 nM and the block is complete and reversible.
With the additional co-expression of either the b1 or b4 subunits, the block become
irreversible or causes the channel to be almost insensitive to the toxin, respectively.1