rProtein A Sepharose Buffer Kit 抗體純化試劑

貨號 Cat. No.
CA000
規格 Size
1 kit
品牌 Brand
gene-rl

Description

抗體純化試劑














rProtein A Sepharose Buffer Kit


(Cat. No. CA000 )


Introduction:


 





rProtein A Sepharose is an affinity medium, designed for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale. The specificity of protein A is primarily for the Fc region of IgG, through which it binds, leaving the antigen binding sites free. However, it can also bind the Fab region through secondary sites. There are differences between the binding affinities for Fc and Fab, usually Fc binding is stronger.


The recombinant protein A used in the manufacture of rProtein A Sepharose has been specially engineered to give very high binding capacities. It is produced in E. coli and purified by a multi step chromatographic procedure. Purification does not involve the use of IgG or any other proteins.


Contents of the kit:










































Items


Quantity


Storage conditions


Dilute notice


Protein Buffer I (CA001), pH9.0


1 bottle ( 1 L )


-20 ℃


 


Protein Buffer II (CA002),pH3.0


1 bottle ( 1 L )


-20 ℃


 


AB Dissolve Buffer (CA003),Ph7.5


1 bottle (100 ml)


-20 ℃


 


Guanidine Hydrochloride Buffer (CB005)


1 bottle (100 ml)


room temperature


 


100x Protease Inhibitor (CB007)


1 vial (1 ml)


-20 ℃


*



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