rOsK-1
- 貨號 Cat. No.
- RTO-150
- 規格 Size
- 5 μg 5x5 μg 10μg 10μgx10μg 0.1mg
Description
rOsK-1
Potent Inhibitor of Kv1.3 and KCa3.1 K+ Channels
| Product#: | RTO-150 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 4205 |
| Sequence: | GVIINVKCKI SRQCLEPCKK AGMRFGKCMN GKCHCTPK |
 | NMR structure of OsK-1 (1SCO).2,3 OsK-1 belongs to the a-KTx3.7 scorpion toxin family. Structure visualized with DS Visualizer (Accelrys). |
| Purity: | >98% |
| Bioassay: |
| rOsK-1 potently inhibits Kv1.3 K+ channels expressed in Xenopus oocytes. |
 |
Left: Current responses to 100 ms depolarization to 0 mV from holding potential of ?00 mV, delivered every 10 seconds before (black) and during application of increasing rOsK-1 concentrations (see inset). Right: Dose response of current inhibition by rOsK-1 (Mean and SD, n=2-6 for each data point). The apparent IC50 is about 10 nM. |
| References: |
1. | Grishin, E.V. et al. (1996) Pure Appl. Chem. 68, 2105. |
2. | Mouhat, S. et al. (2005) Biochem. J. 385, 95. |
3. | Jaravine, V.A. et al. (1997) Biochemistry 36, 1223. |
OsK1 (a-KTx3.7) is a 38-residue toxin cross-linked by three disulphide bridges, originally isolated from the venom of the Asian scorpion Orthochirus scrobiculosus.1 The toxin was found to block a number of K+ channels, both voltage-dependent and Ca2+-activated isoforms. OsK-1 is lethal in mice by intracerebroventricular injection, with a LD50 value of 2 mg/kg. OsK-1 blocks mKV1.1, mKV1.2, mKV1.3 channels potently and hKCa3.1 channel moderately, with IC50 values of 0.6, 5.4, 0.014 and 225 nM, respectively.2 No inhibition effects were observed on KV1.4, KV1.5, KV1.6, KV1.7, KV11, KCa2.1, KCa2.2, KCa2.3 and KCa1.1 channels at mM concentrations. 2