Romosozumab ELISA Kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
- Application 應用
- Used for the quantitative determination of Romosozumab concentration in serum and plasma.
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human SOST has been pre-coated onto a microplate. Standards or samples are premixed with
biotin-labeled antibody and then pipetted into the wells. Romosozumab in the sample competitively binds to the pre-coated protein with biotin-labeled Romosozumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Romosozumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human SOST has been pre-coated onto a microplate. Standards or samples are premixed with
biotin-labeled antibody and then pipetted into the wells. Romosozumab in the sample competitively binds to the pre-coated protein with biotin-labeled Romosozumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Romosozumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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