ROCK-1 (cleaved) Antibody

Antibody Target :ROCK-1 (cleaved)

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide corresponding to the caspase-3 cleavage site

Accession#: Q13464

Gene ID: 6093

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) Protein G purified mouse anti-human ROCK1 monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms

Application: WB

Application & Usage: Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate treated with camptothecin (2 µM for 6-8 hours) can be used as a positive control. The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.