rNoxiustoxin

rNoxiustoxin

A Potent Blocker of Some K_v1 Channels

Product#:

RTN-340

Sizes:

5 mg

5 x 5 mg

10 mg

5 x 10 mg

0.1 mg

Noxiustoxin was originally isolated from the scorpion Centruroides noxius Hoffmann¹ and was shown to interact and inhibit K_v1.2 and K_v1.3 K⁺ channels.² The toxin blocks K_v1.3 (as assessed by whole cell recording from Jurkat cells) with K_d of 6 nM.³  It was shown to bind to synaptosomal membranes⁴ and to increase transmitter release.⁵ In addition it was shown to block K⁺ currents in lymphocytes.⁶

M.W.:

4195

Sequence:

TIINV KCTSP KQCSK PCKEL YGSSA GAKCM NGKCK CYNN

Purity:

>99%

Bioassay:

rNoxiustoxin inhibits Kv1.3 channels expressed in Xenopus oocytes.

Left: Time course of Kv1.3 current amplitude in response to test pulse of 100 ms to 0 mV (holding potential -100 mV). 200 nM rNoxiustoxin were perfused at the period marked by the horizontal bar. Right: Traces of Kv1.3 currents before (red) and during application of the toxin.

References:

1.	Possani, L.D. et al. (1982) Carlsberg Res. Commun. 47, 285.
2.	Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227
3.	Mullmann, T.J. et al. (2001) Biochemistry 40, 10987.
4.	Martinez, F. et al. (1996) Toxicon 34, 1413.
5.	Sitges, M. et al. (1986) J. Neurosci. 6, 1570.
6.	Drakopoulou, E. et al. (1995) Biochem. Biophys. Res. Commun. 213, 901.