rMaurocalcine
- 貨號 Cat. No.
- RTM-100
- 規格 Size
- 5 μg 5x5 μg 10μg 10μgx10μg 0.1mg
Description
rMaurocalcine
Ryanodine receptor 1 and 2 modulator
| Product#: | RTM-100 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 3864 |
| Purity: | >98% |
| Bioassay: |
| rMaurocalcine prolongs the duration of spontaneous long lasting Ca2+ release events in cardiomyocyte cell culture. |
 |
High density cardiomyocyte cell culture prepared from hearts of 3-4 days old rat pups were used 3 days after seeding (under these conditions the culture is almost synchronized). The cells were loaded with 4 mM fluo-3 AM and the Ca2+ traces were measured before (blue) and after (red) stimulation with 400nM rMaurocalcine (#RTM-100). rMaurocalcine evokes long lasting Ca2+ release events in skeletal myotubes. |
 |
| Myotube cell cultures were prepared from limbs of 2 days rat pups and were loaded with 4 mM fluo-3 AM and then stimulated with rMaurocalcine. The photograph shows Ca2+ spread in the same myotube before (A) and after (B) stimulation with 200nM rMaurocalcine (#RTM-100). |
| References: |
1. | Fajloun, Z. et al. (2000) FEBS Lett. 469, 179. |
2. | Mosbah, A. et al. (1998) 6eme Rencontre en Toxinologie, Soci彋?fran蓷is pour l'etude des toxines, Paris. August 1-4. |
3. | Est鋦e, E. et al. (2005) J. Biol. Chem. 280, 12833. |
4. | Chen , L., et al. (2003) J. Biol. Chem. 278, 16095. |
5. | Tripathy, A. et al. (1998) J. Gen. Physiol. 111, 679. |
Maurocalcine (MCa), is a peptidyl toxin originelly isolated from the venom of the scorpion Scorpio maurus palmatus.
Native MCa is present at a low concentration in the venom (0.5% of total protein).This 33-mer basic peptide contains three disulfide bridges and has 82% sequence homology to Imperatoxin A (IpTxa), a scorpion toxin from the venom of Pandinus imperator. MCashares the highly basic structural domain identified in peptideA - a segment of the Dihydropyridine Receptor and IpTxa which is important for interactions with Ryanodine receptors. MCa also appears to share the same features as the so-calledcell-penetrating peptides which allows the toxin to efficiently penetrate various cell types without expending metabolic energy or using an endocytoticmechanism.1-4
In vitro, electrophysiological experiments based on recordings of single channels incorporated into planar lipid bilayers showed that MCa potently and reversibly modifies channel gating behavior of the ryanodine receptor 1 (RR1) by inducing prominent subconductance behavior.1,2
Similar to IpTxa, MCa-modified channels can reversibly shuttle between subconductancestates and fast gating states. The actions of these peptides werealso found to be additive with those of ryanodine, resulting inadditional sub-states along with the ryanodine-locked half-conductingstate. However, the predominant subconductance induced by MCadiffers from that of IpTxa. At a holding potential of +40 mV,the predominant substates induced by sMCa and IpTxa are 48 and28% of the full conductance, respectively, suggesting that thestructural difference in these peptides may induce slightly differentchannel conformations that stabilize different unitary conductances.5
Alomone Labs is pleased to offer rMaurocalcine, a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.