rKaliotoxin-1
- 貨號 Cat. No.
- RTK-370
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rKaliotoxin-1
A Powerful Blocker of Certain Kv1and KCa1.1 Channels
| Product#: | RTK-370 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 4022 |
| Sequence: | GVEINVKCSG SPQCLKPCKD AGMRFGKCMN RKCHCTPK |
 | NMR structure of Kaliotoxin (1KTX). 6,7 Kaliotoxin belongs to the a-KTX-3.1 scorpion toxin family.2 |
| Purity: | >99% |
| Bioassay: |
Inhibition of K V1.3 channels expressed in Xenopus oocytes by rKaliotoxin-1. |
 |
Left: Time course of current amplitude upon 250 nM rKaliotoxin-1 application and wash. Oocytes were held at ?00mV holding potential using two electrode voltage clamp. Currents were elicited by 100 ms test pulse to 0 mV, delivered every 10 seconds. Inhibition was completely reversible. Toxin was applied at the period indicated by the horizontal bar. Middle: Traces of current response before (red) and during application of 250 nM toxin. Right: Dose response curve for K V1.3 channels inhibition. Each point represents 2-4 experiments as mean and S.D. |
| References: |
1. | Crest, M. et al. (1992) J. Biol. Chem. 267, 1640. |
2. | Rodriguez de la Vega, R.C. and Possani, L.D. (2004), Toxicon 43, 865. |
3. | Mourre, C. et al. (1999) J. Pharmacol. Exp .Ther. 291, 943. |
4. | Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227. |
5. | Hopkins, W.F. et al. (1996) Curr. Pharmaceut. Design. 2, 389. |
6. | Gairi, M. et al. (1997) J. Pept. Sci. 3, 314. |
7. | Fernandez, I. et al. (1994) Biochemistry 33, 14256. |
Kaliotoxin-1 is 38 amino acid long toxin, originally isolated from the venom of the scorpion Androctonus mauretanicus mauretanicus and is classified as a-KTX 3.1 scorpion toxin family, having three disulfide bridges.1,2 Kaliotoxin-1 is a potent inhibitor of large conductance Ca2+ -activated K+ channels (KCa1.1), and to bind and inhibit Dendrotoxin sensitive voltage-dependent K+ channels, mainly Kv1.1, Kv1.2 and Kv1.3 (KCNA1 KCNA2 and KCNA3) with Kd of 1.5, 25 and 0.1nM, respectively.1,3,4,5