- 貨號 Cat. No.
- RTH-400
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rHongotoxin-1
A Potent Blocker of Some KV1 K+ Channels
Sizes: |
5 mg |
5 x 5 mg |
10 mg |
5 x 10 mg |
0.1 mg |
|
Sequence: |
TVIDV KCTSP KQCLP PCKAQ FGIRA GAKCM NGKCK CYPH |
Bioassay: |
The recombinant toxin blocks Kv1.3 channels heterologously expressed in Xenopus oocytes, with IC50 of 5 nM.
|
 |
Kv1.3 channel current (in 2mM K+), elicited by 100 ms depolarization from holding potential of ?00 mV to 0 mV, before and during application of the indicated rHongotoxin-1 concentration. |
1.
|
Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639.
|
2.
|
Legros, C. et al. (2000) J. Biol. Chem. 275, 16918. |
Hongotoxin was originally isolated from the scorpion Centruroides limbatus1 and was shown to block cloned and heterologously expressed (in HEK 293 cells) Kv1.1, 1.2 and 1.3 with IC50 of 31, 170 and 86 pM respectively.1 In addition, it blocks Kv1.6 with low affinity (IC50 = 6nM). rHongotoxin-1 action was examined by monitoring radiolabeled Rb+ efflux from Kv channels-transfected HEK 293 cells. rHongotoxin-1 blocks 125I-Margtoxin (Margatoxin) binding to rat brain synaptosomes1 or 125I-Kaliotoxin binding to purified Kv1.3-KcsA chimeras.2 A mutated 125I-Hongotoxin-1 was used to immunoprecipitate (with specific Kv antibodies) rat brain Kv channels and to determine their subunit composition.1
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