Rhodamine Red-His6-Apg8p2 (GATE16)
- 貨號 Cat. No.
- UL-426
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Rhodamine Red-His6-Apg8p2 (GATE16)
human recombinant
Product Information
50 μg
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
15.7 kDa
> 95% by PAGE
Use & Storage
Rhodamine Apg8p2 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex570 nm and Em590 nm wavelengths respectively.
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin by washing with 5 ml 50 mM Hepes pH 8.0, 1 M NaCl. Remove cleaning solution by washing resin with 10 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.
Catalog #: UL-426
Rhodamine Red-His6-Apg8p2 (GATE16)
Product Source:
human recombinant
His6-Apg8p2 modified with rhodamine red via primary amine coupling. This results in rhodamine coupled Apg8p2 species with modified lysines as well as the N-terminus. Detection of rhodamine-Apg8p2 occurs with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW:
15.7 kDa
Purity:
> 95% by PAGE
Use & Storage
Use:
Rhodamine Apg8p2 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex570 nm and Em590 nm wavelengths respectively.
Storage:
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin by washing with 5 ml 50 mM Hepes pH 8.0, 1 M NaCl. Remove cleaning solution by washing resin with 10 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.