rHeteropodatoxin-2
- 貨號 Cat. No.
- RTH-340
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rHeteropodatoxin-2
A Novel Kv4 K+ Channel Blocker
| Product#: | RTH-340 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
Native Heteropodatoxin-2 was originally isolated from the Heteropoda venatoria spider venom.
rHeteropodatoxin-2 is a recombinant peptide expressed in and extracted from
E. coli and purified to homogeneity. Native Heteropodatoxin-2 blocked Ito1 in ventricular myocytes
in a voltage dependent manner with IC50 (at ?FONT size=2>10 mV) of 16 nM.
The toxin also blocked KV4.2 expressed in Xenopus oocytes in a similar manner.1
E. coli and purified to homogeneity. Native Heteropodatoxin-2 blocked Ito1 in ventricular myocytes
in a voltage dependent manner with IC50 (at ?FONT size=2>10 mV) of 16 nM.
The toxin also blocked KV4.2 expressed in Xenopus oocytes in a similar manner.1
| M.W.: | 3414 |
| Sequence: | DDCGK LFSGC DTNAD CCEGY VCRLW CKLDW |
| Purity: | >98% |
| Bioassay: |
| The biological activity of rHeteropodatoxin-2 was tested using two-electrode voltage clamp recording from Xenopus oocytes expressing the KV4.2 channels. |
 |
| rHeteropodatoxin-2, inhibition of KV4.2 channels expressed in Xenopus oocytes. Middle: Current responses to 100 ms depolarization to 0 mV from holding potential of ?00 mV, delivered every 10 seconds before (red) and during application of 500 nM rHeteropodatoxin-2. Left: Time course of amplitude inhibition and wash. Right: I-V relationships for KV4.2 before and during rHetropodatoxin-2, perfusion. Also included, the fraction of inhibited current as function of the voltage step (see inset for symbols) to demonstrate the voltage dependency of block. |
| References: |
1. | Sanguinetti, M. C. et. al. (1997) Mol. Pharmacol. 51, 491. |