Rhesus macaque / Cynomolgus Complement Factor D / CFD Protein, His Tag

Source : 

Rhesus macaque / Cynomolgus Complement Factor D, His Tag (CFD-R52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Ala 253 (Accession # H9EXC1-1). In the region Ile 26 - Ala 253, the AA sequence of Rhesus macaque and Cynomolgus Complement Factor D are homologus.

 

Molecule : Complement Factor D

 

Synonyms : CFD,Adipsin,PFD,DF,Complement factor D

 

Format : Powder

 

Category : Enzymes

 

Accession : XP_005587397.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 26.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 25-27 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH8.0

 

Description :

Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.

 

References :

(1) White R.T., et al., 1992, J. Biol. Chem. 267:9210-9213.
(2) Johnson D.M.A., et al., 1984, FEBS Lett. 166:347-351.
(3) Sprong T., et al., 2006, Blood 107:4865-4870.