RFP (dsRed) Monoclonal Ab

HOST: Mouse IgG1 (Clone# BV-F9)

IMMUNOGEN: Recombinant Red Fluorescence Protein (dsRed) expressed from E. coli (Cat.# 4997-100) SPECIES: All

FORMULATION: 100 ug (0.5 mg/ml) antigen affinity purified mouse anti-RFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

BACKGROUND: Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

SPECIFICITY: The antibody reacts with RFP. Recombinant RFP (Cat.# 4997-100) can be used as a positive control.

APPLICATION AND USAGE: The antibody can be used for Western blotting (0.5-4 ug/ml), immunofluorescence (10-20 ug/ml) and immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.

STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.