RFP (dsRed) Antibody

Antibody Target: RFP (dsRed)

Alternative Name: Red Fluorescent Protein

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Recombinant RFP expressed from E. coli

Accession#: P00273

Gene ID: 3229046

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-RFP polyclonall antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: all

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). Based on researchers’ feedback, it can also be used in immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Handling: The antibody solution should be gently mixed before use.