Antibodies
Resveratrol
- 貨號 Cat. No.
- S1396
- 規格 Size
- 10mM/1mL~500mg
- 品牌 Brand
- Selleckchem
Description
Resveratrol
Resveratrol is a phytoalexin produced naturally by several plants with anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | Resveratrol is a phytoalexin produced naturally by several plants with anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular effects. | |||||
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| Targets | COX | LOX | DNA polymerasesα | DNA polymerasesδ | IKK β | Quinone reductase 2 |
| IC50 | 15 μM | 3.7 μM | 3.3 μM | 5 μM | 1 μM | 35-50 nM [1] |
| In vitro | Resveratrol inhibits the activity of Cyclooxygenase and lipooxygenase, PKCs and p56lck, ERK1, JNK1, p38, IKK β, Src, STAT3, Ribonucleotide Reductase, DNA polymerases α and δ, PKD, PKC α, Quinone reductase 2, and Aromatase with IC50 of from 0.035-60 μM. Resveratrol is also an activator of Adenylyl cyclase and AMPK with EC50 of 0.8 μM and 50 μM, respectively. The roles of Resveratrol as inhibitor or activator enable its effects on decreasing cell inflammatory associated behaviors, growth inhibition and induction of apoptosis in cancer cells, reversal of endothelin-1 stimulated cell responses, inhibition of phorbol ester-induced expression of COX-2, inhibition of DNA synthesis in cell, resistance to menadione-induced cell death, and improvement of cell mitochondrial function and glucose/lipid metabolism. [1] Resveratrol is also an activator of sirtuins. Resveratrol lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD+, and increases cell survival by stimulating SIRT1-dependent deacetylation of p53. In yeast, Resveratrol mimics calorie restriction by stimulating Sir2, increasing DNA stability and extending lifespan. [2] Resveratrol is effective at protecting isolated rat hearts against ischemia/reperfusion injury via its antioxidant activity, with improved recovery of developed pressure and aortic flow, reduction of malondialdehyde concentrations and reduction of infarct size. [3] | |||||
| In vivo | Resveratrol improves health and survival of mice on a high-calorie diet. Resveratrol (22.4 mg/kg/day) shifts the physiology of middle-aged mice on a high-calorie diet towards that of mice on a standard diet and significantly increases their survival. Resveratrol produces changes associated with longer lifespan, including increased insulin sensitivity, reduced insulin-like growth factor-1 (IGF-I) levels, increased AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α) activity, increased mitochondrial number, and improved motor function. Resveratrol opposed the effects of the high-calorie diet in 144 out of 153 significantly altered pathways. [3] Resveratrol has been shown to inhibit the initiation and growth of tumors in a wide variety of rodent cancer models. Dose of Resveratrol as low as 200 μg/kg daily already shows efficacy in a rat model of colon carcinogenesis. At higher dose of 40 mg/kg, Resveratrol increases the survival of mice with subcutaneous neuroblastomas from 0% to 70%. Resveratrol inhibits vascularization in the corneal micropocket assay in mice at a dose of only 48 μg/kg when administered daily. [3] Resveratrol shows beneficial effects on heart disease. Resveratrol blocks the increase in platelet aggregation induced by a hypercholesterolaemic diet. Resveratrol increase expression of both endothelial and inducible nitric oxide synthase. In stroke-prone, spontaneously hypertensive rats, resveratrol significantly reduces markers of oxidative stress such as glycated albumin in serum, and 8-hydroxyguanos-ine in urine. Providing Resveratrol in drinking water for 15 days (1 mg/kg) is sufficient to improve the recovery in function and coronary flow of isolated hearts. [3] Resveratrol displays anti-inflammatory activity in vivo. Resveratrol significantly reduces both acute and chronic chemically induced oedema, lipopolysaccharide-induced airway inflammation and osteoarthritis, and helps to prevent allograft rejection. Intravenously administered Resveratrol decreases inflammation induced by ischaemia/reperfusion, oxidants generated by hypoxanthine/xanthine oxidase (HX/XO) or platelet-activating factor, but not leukotriene B4 in rats. [3] Resveratrol shows beneficial effects on stroke and brain damage. Resveratrol administered intravenously significantly decreased ischaemic volume and brain water content at the extremely low doses of 100 ng/kg and 1 μg/kg after middle cerebral artery occlusion in rats. [5] | |||||
| Features | ||||||
Protocol(Only for Reference)
Cell Assay: [6]
| Cell lines | Human breast cancer cell MCF-7 |
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| Concentrations | 30-300 μM |
| Incubation Time | 2 days |
| Method | Cells are plated in flat-bottomed, 96-well microtiter plates (4000 cells/6.4-mm-diameter well). After 12–24 hr, cells are treated with DMSO (0.1–0.3%) or increasing doses of Resveratrol. After 48 h of treatment, cells are treated with 10μL of MTT reagent for 4 hr at 37 ℃ and then treated with 100 μL of solubilization solution at 37 ℃ overnight. The quantity of formazan product is measured using a spectrophotometric microtiter plate reader at 570 nm wavelength. |
Animal Study: [7]
| Animal Models | Human ovarian xenografts PA-1 |
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| Dosages | 100 mg/kg |
| Administration | i.p. daily for consecutive 4 weeks |
| Solubility | 30% PEG400/0.5% Tween80/5% propylene glycol, 30 mg/mL |
References
Clinical Trial Information( data from http://clinicaltrials.gov)
| NCT Number | Recruitment | Conditions | Sponsor /Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT01881347 | Recruiting | Diabetes Mellitus | Boston University|National Heart, Lung, and Blood Institute (NHLBI) | 2013-06 | |
| NCT01914081 | Not yet recruiting | Non-ischemic Cardiomyopathy | St. Boniface General Hospital Research Centre|Canadian Centre for Agri-food Research in Health and Medicine|Agriculture and Agri-Food Canada|Manitoba Medical Service Foundation | 2013-09 | Phase 3 |
| NCT01964846 | Recruiting | Effect of Resveratrol and Curcumin on Inflammation | Laval University|Atrium Innovations | 2013-10 | |
| NCT01997762 | Not yet recruiting | Gestational Diabetes | University of Manitoba|Manitoba Institute of Child Health | 2013-12 | Phase 4 |
| NCT02001831 | Not yet recruiting | Physical Disability | University College Dublin|Smartfish AS | 2014-01 |
Chemical Information
| Molecular Weight (MW) | 228.24 |
|---|---|
| Formula | C14H12O3 |
| CAS No. | 501-36-0 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | N/A |
| Solubility (25°C) * | In vitro | DMSO | 46 mg/mL (201 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | <1 mg/mL (<1 mM) | ||
| In vivo | 30% PEG400/0.5% Tween80/5% propylene glycol, | 30 mg/mL | |
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | (E)-5-(4-hydroxystyryl)benzene-1,3-diol |
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Preparing Stock Solutions
| Stock Solution (1ml DMSO) | 1mM | 10mM | 20mM | 30mM |
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| Mass(mg) | 0.22824 | 2.2824 | 4.5648 | 6.8472 |
Customer Reviews (1)
 Click to enlarge | Rating | |
| Source | Bai Bo University of Hong Kong. Resveratrol purchased from Selleck | |
| Method | Western Blot | |
| Cell Lines | PAECs | |
| Concentrations | 100 μM | |
| Incubation Time | 24 h | |
| Results |
