Antibodies
Re-ChIP-IT
Kits
- 貨號 Cat. No.
- 53016
- 規格 Size
- 25 rxns
- 品牌 Brand
- Active motif
Description
Identify protein co-localization in vivo with Re-ChIP-IT
Chromatin immunoprecipitation (ChIP) is extremely useful for studying chromatin biology and transcriptional regulation because it enables the localization of chromatin proteins, modified histones and transcription factors that are bound to specific DNA regions in specific cells. Furthermore, because protein/DNA interactions are fixed while in an endogenous, chromosomal context, ChIP results reflect the influence of chromosomal topology and the effects of cellular regulatory proteins.
The Re-ChIP-IT® Kit makes it possible to identify the simultaneous binding of two transcription factors or two histone modifications on a DNA fragment of interest. Chromatin that has been immunoprecipitated is removed from the magnetic beads with a special buffer that prevents the majority of the first antibody from participating in the second IP reaction. The chromatin is then desalted and a second ChIP is performed using a different antibody from the first. The cross-links of these sequentially immunoprecipitated protein-DNA complexes are then reversed and the DNA is analyzed by PCR, similar to conventional ChIP samples.
Chromatin immunoprecipitation (ChIP) is extremely useful for studying chromatin biology and transcriptional regulation because it enables the localization of chromatin proteins, modified histones and transcription factors that are bound to specific DNA regions in specific cells. Furthermore, because protein/DNA interactions are fixed while in an endogenous, chromosomal context, ChIP results reflect the influence of chromosomal topology and the effects of cellular regulatory proteins.
The Re-ChIP-IT® Kit makes it possible to identify the simultaneous binding of two transcription factors or two histone modifications on a DNA fragment of interest. Chromatin that has been immunoprecipitated is removed from the magnetic beads with a special buffer that prevents the majority of the first antibody from participating in the second IP reaction. The chromatin is then desalted and a second ChIP is performed using a different antibody from the first. The cross-links of these sequentially immunoprecipitated protein-DNA complexes are then reversed and the DNA is analyzed by PCR, similar to conventional ChIP samples.