rCNTF
- 貨號 Cat. No.
- C-245
- 規格 Size
- 5 μg 5x5 μg
Description
rCNTF
Rat Ciliary Neurotrophic Factor
| Product#: | C-245 |
| Sizes: |
| 5 µg |
| 5 x 5 µg |
| Preparation: |
| rCNTF is a recombinant growth factor produced in E. coli. |
| M.W.: |
| 22.7 kDa (200 amino acids). |
| Purity: |
| Greater than 98% pure as estimated by SDS-PAGE and HPLC analysis. |
| Bioassay: |
| CNTF attenuates the activation of STAT3 and ERK differently in adipocytes and preadipocytes 3T3 L1 cells. |
 | Preadipocytes 3T3-L1 cells or adipocytes 3T3-L1 cells were stimulated with increased concentrations of rCNTF (#C-245) for 10 min. The cells were subjected to Western blot analysis and the activation of ERK and Stat 3 were determine by western blot using anti phospho-ERK and anti phospho-Stat 3. |
| Locus Link ID: | 25707 CNTF. |
| References: |
1. | Mufson, E.J. et al. (1999) Prog. Neurobiol. 57, 451. |
2. | Adler, R. et al. (1979) Science 204, 1434. |
3. | Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355. |
4. | Henderson, J.T. et al. (1994) Mol. Brain Res. 22, 151. |
5. | Stahl, N. et al. (1993) J. Biol. Chem. 268, 7628. |
6. | Richardson, P.M. (1994) Pharmacol. Ther. 63, 187. |
7. | Ip, N.Y. et al. (1991) J. Physiol. 85, 123. |
8. | Gurney, M.E. et al. (1992) J. Neurosci. 12, 3241. |
9. | Sendtner, M. et al. (1992) Nature 358,502. |
10. | Mayer, M. et al. (1994) Development 120, 143. |
CNTF is a polypeptide hormone, member of the alpha-helical cytokine superfamily.1 It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture.2 CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain.4 CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitoryfactor receptor, gp130) and a CNTF-specific ligand-binding-subunit(CNTFR).5
CNTF can support the survival of many different cell populations within the PNS and the CNS.6 CNTF in vitro, promote proliferation and neuronal specificationin in hippocampal neurons. It supports the viability of non-primate motor neurons,7 In vivo, CNTF induces sprouting of cholinergic motor neurons8and delays neural degeneration in genetic models of motor neuron disease.9 In addition, it is involved in the development stage of astrocytes and oligodendocytes.10
CNTF can support the survival of many different cell populations within the PNS and the CNS.6 CNTF in vitro, promote proliferation and neuronal specificationin in hippocampal neurons. It supports the viability of non-primate motor neurons,7 In vivo, CNTF induces sprouting of cholinergic motor neurons8and delays neural degeneration in genetic models of motor neuron disease.9 In addition, it is involved in the development stage of astrocytes and oligodendocytes.10
Alomone Labs is pleased to offer CNTF, a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.