rCharybdotoxin

貨號 Cat. No.

RTC-325

規格 Size

5 μg 5x5 μg 10μg 5x10μg 0.1mg

Description

rCharybdotoxin

A Potent Blocker of Some Ca²⁺ and Voltage Dependent K⁺ Channels

Product#:

RTC-325

Sizes:

5 mg

5 x 5 mg

10 mg

5 x 10 mg

0.1 mg

Charybdotoxin was originally isolated from the venom of the Israeli scorpion Leiurus quinquestriatus hebraeus. Charybdotoxin blocks K_Ca1.1 (large conductance Ca²⁺ activated K⁺, Slo) channels in nanomolar concentrations² as well as K_v1.2 (Kd, 14 nM) and K_v1.3 (Kd, 2.6 nM) channels.³ However, experiments with cloned K_Ca1.1 channels demonstrate the strong effect of the slob subunits on the potency of block by Charybdotoxin (see for example⁴).

M.W.:

4313

Sequence:

QFTNV SCTTS KECWS VCQRL HNTSR GKCMN KKCRC YS

Purity:

>99%

Bioassay:

This toxin was tested to confirm its ability to potently inhibit Kv1.3 K+ channels expressed in Xenopus oocytes. The IC50 in this assay was 1nM.5

The effect of 100 nM rCharybdotoxin on KCa1.1 (left) and Kv1.3 (right, co-expressed with Kvb1.1) channels, expressed in Xenopus oocytes. In both cases the currents were recorded with standard ND96 solution (see above), Membrane holding potential was ?00 mV, stepped every 15 s. to +20 mV for 100 ms. The vertical bar represents 0.1 and 3 mA for KCa1.1 and Kv1.3 respectively.

References:

1.	Gimenez-Gallego, G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 3329.
2.	Miller, C.E. et al. (1985) Nature 313, 316.
3.	Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227.
4.	Meera, P. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5562.
5.	Sugg, E.E. et al. (1990) J. Biol. Chem. 265, 18745.

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