rBeKm-1
- 貨號 Cat. No.
- RTB-470
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rBeKm-1
A Novel ERG1 (Kv11.1) K+ Channel Blocker
| Product#: | RTB-470 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 4092 Da |
| Sequence: | RPTDI KCSES YQCFP VCKSR FGKTN GRCVN GFCDC F |
| Purity: | >98% |
| Bioassay: |
| Electrophysiological recordings demonstrating the effects of nM concentrations of rBeKm-1 on KV11.1 (hERG) channels expressed in Xenopus oocytes and HEK-293 cells. |
 |
rBeKm1 toxin at 50 nM reduced KV11.1 (hERG1) channels current in Xenopus oocytes by 90% (above). Kv11.1 channel responses to 200 ms pulses from holding potential ofmA. ?FONT size=2>100 mV to + 20 mV, before and during application of 50 nM rBeKm1, with 2 mM K+ as charge carrier. The vertical bar represents 1 |
 |
Effects of rBeKm-1 on KV11.1 (hERG) channels stably expressed in HEK-293 cells using whole cell patch clamp recording (see two graphs above). Top: Traces of KV11.1 currents elicited with the voltage protocol that is illustrated below, for control currents and increasing toxin concentrations (details in the inset). Bottom: Dose response for the toxin effect in these cells (n=2-7 for each data point, IC50 of about 2 nM). |
| References: |
1. | Korolkova, Y.V. et al. (2001) J. Biol. Chem. 276, 9868. |
2. | Filippov, A.K. et al. (1996) FEBS Lett. 384, 277. |
BeKm-1 was originally isolated from Mesobuthus eupeus scorpion venom. rBeKm-1 is a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity. BeKm-1 toxin blocked hERG1 channels expressed in HEK 293 cells with IC50 of 3.3 nM.1 The native toxin blocked M currents in differentiated mouse neuroblastoma X rat glioma NG108-15 cells with IC50 of 33 nM.2