rATX II
- 貨號 Cat. No.
- RTA-700
- 規格 Size
- 50 μg 5x50 μg 0.1mg 5x0.1mg 0.1mg
Description
rATX II
Voltage-gated Na+ Channel Modulator
| Product#: | RTA-700 |
| Sizes: |
| 50 mg |
| 5 x 50 mg |
| 0.1 mg |
| 5 x 0.1 mg |
| M.W.: | 4935 |
| Sequence: | GVPCL CDSDG PSVRG NTLSG IIWLA GCPSG WHNCK KHGPT IGWCC KQ |
| Purity: | >99% |
| Bioassay: |
 |
Dose dependent enhancement of TTX-Sensitive NaV currents by rATX-II. (see above) Left: Time course of NaV current amplitude responses to voltage step stimulation in ND7-23 cells. Under patch clamp whole cell, holding potential was set to ?00 mV and 40 ms pulses to ?0 mV were delivered every 10 seconds. Current amplitude was measured 5 ms after the onset of the test pulse (during inactivation). Increasing concentrations of rATX-II (#RTA-700) were perfused and washed in the following order: 50 nM, 250 nM, 500 nM, 1 mM, 2 mM and 5 mM, these periods are indicated by the bars. Middle: Superimposed traces of current responses before (green) and during (black) application of 1 mM rATX-II. Right: A summary dose response curve taken from four experiments such as the one described on the left (mean ?SD). |
 |
Potent enhancement of NaV1.5 channel currents by rATX-II. (see above)
Right: Dose response curve for rATX-II acting on NaV1.5 currents (mean ?SD, n=7-12 experiments for each point) expressed as fold increase in current amplitude, measured 5 ms after the onset of the test pulse (inactivation period). Left: Example traces of NaV1.5 channel currents responses to a 40 ms test pulse (-30 mV, holding potential ?00 mV) before (blue) and during (purple) application of 10 nM rATX-II (#RTA-700). Currents were recorded from HEK-293 cells, stably expressing NaV1.5 channels. |
| References: |
1. | Romey, G. et al. (1976) Proc Natl. Acad. USA 73, 4055. |
2. | Tesseraux, I. et al. (1987) Naunyn Schmiedebergs Arch. Pharmacol. 336, 232. |
3. | Nishio, M. et al. (1991) Br. J. Pharmacol. 104, 504. |
4. | Cannon, S.C. and Corey D.P. (1993) J. Physiol. 466, 501. |
5. | Hoey, A. et al. (1994) Pharmacol. Toxicol. 75, 356. |
6. | Chahine, M. et al. (1996) J. Membr. Biol. 152, 39. |
7. | Fletcher, J. E. et al. (1999) Anesthesiology 90, 1294. |
8. | Brand, S. et al. (2000) Eur. J. Neurosci. 12, 2387. |
9. | Ravens, U. (1976) Naunyn-Schmiedebergî–¸ Arch. Pharmacol. 296, 73. |
rATX II is a 47 amino acid peptidyl toxin, originally isolated from Anemonia sulcata sea anemone venom. It is a potent neurotoxin, which modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types (at concentration range of 10-100nM).1-9