Rat TNF-alpha

SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses.

Endotoxin level is <0.1 ng per ìg of TNF-á.

MOL. WEIGHT: 17.0 kDa

FORMULATION: Sterile filtered and lyophilized from 1X PBS

RECONSTITUTION:

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4^oC for 1 week or –20^oC for future use.

STORAGE CONDITIONS:

The lyophilized TNF-á is best-stored desiccated below 0^oC. Reconstituted TNF-á should be stored at working aliquots at -20^oC.

DESCRIPTION:

Tumor Necrosis Factor-alpha (TNF-á) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-á is a 17 kDa protein containing 157 amino acid residues.

BIOLOGICAL ACTIVITY:

The _ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 10⁷ units/mg.

RECOMMENDED USAGE:

For most in vitro applications, TNF-á exerts its biological activity in the concentration range of 0.5 to 20 ng/ml.

FOR RESEARCH USE ONLY! Not to be used in human subjects!