Rat NGF (NGF beta) ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Nerve growth factor; beta-NGF;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1386

 

Description (Long Description) : 

The rat NGF Kit is a sandwich ELISA. The capture antibody is a monoclonal rat NGF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known NGF concentration are added to these wells and allowed to complex with the bound NGF antibody. A biotinylated rat NGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat NGF in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids S122-G241

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :