rat Leptin

SOURCE: E. coli

PURITY: >95% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of Leptin.

MOL. WEIGHT: 16.2 kDa

FORM: Lyophilized from 4.5 uM NaHCO3.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

STORAGE CONDITIONS: The lyophilized protein is best stored at -20oC. Reconstituted Leptin should be stored in working aliquots at -20oC. Avoid freeze/thaw cycles.

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.

USAGE: For Research Use Only. Not for use in diagnostic or therapeutic procedures