Rat Glial cell line-derived neurotrophic factor/GDNF/ATF ELISA(1 plate)

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor 1; ATF-1

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1331

 

Description (Long Description) : 

The rat GDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-rat GDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known GDNF concentration are added to these wells and allowed to complex with the bound GDNF antibody. A biotinylated ant-rat GDNF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GDNF present in the samples. The purpose of this kit is for quantitative detection of rat GDNF in cell culture supernatants, cell lysates, tissue homogenates, serum, and heparin or EDTA treated plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen used in this kit is recombinant mature (aa78-211) rat GDNF produced in and purified from an insect cell expression system. This assay will not distinguish between unprocessed proGDNF and mature GDNF as the antibodies will detect both forms when present.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :