Rat Complement C5 Protein, His Tag

Source : 

Rat Complement C5, His Tag (CO5-R52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).

 

Molecule : Complement C5

 

Synonyms : Complement C5,C5,CPAMD4

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : XP_001079130.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 74.1 kDa (β chain) & 114.8 kDa (α chain)

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus.
The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

 

References :

(1) DeMartino JA, et al. 1994. J Biol Chem. 269(20):14446-50.
(2) Fredslund F, et al. 2008. Nat Immunol. 9(7):753-60.
(3) Chen Z, et al. 1998. J Biol Chem. 273(17):10411-9.