Rat CNTF ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Ciliary neurotrophic factor; CNTF

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of rat CNTF in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate) Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1676

 

Description (Long Description) : 

The Rat CNTF ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CNTF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CNTF concentration are added to these wells and allowed to complex with the bound CNTF antibody. A biotinylated CNTF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CNTF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CNTF in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant Rat CNTF produced in E.coli cells; Immunogen sequence: A2-M200.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, rat CNTF protein standards, Rat CNTF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1676