RasGAP Antibody

Antibody Target :RasGAP

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide surrounding amino acid 1025 of human RasGAP

Accession#: P20936

Gene ID: 5921

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt/bv

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes ~120 kDa Ras GAP of human, mouse, rat, and bovine origins.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: The mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTPbinding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.