Proteins and Peptides
RAF1 Antibody (LS-B1487)
- 貨號 Cat. No.
- LS-B1487
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1487 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C34245. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Serine/Threonine Protein Kinase RAF (RAF1) |
| Synonyms: | RAF1, c-raf-1, Raf, Raf1, raf1 kinase, serine/threonine-protein kinase raf, v-raf-1 murine leukemia viral oncogene homolog 1 |
| Family: | Protein Kinase |
| Subfamily: | Raf |
| Host: | RAF1 antibody was produced in Rabbit |
| Antigen Species: | RAF1 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | RAF1 antibody was raised against synthetic peptide |
| Antibody Specificity: | Amino acids surrounding Ser 259 of human RAF1 |
| Antigen Modification: | Ser259 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, 0.02% sodium azide, 50% glycerol. |
| Recommended Storage: | Long term: -70°C; Short term: -20°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1487 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
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