RAD51 Antibody (LS-B2597)

貨號 Cat. No.: LS-B2597
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2597
Antibody Validation:	This antibody replaces catalog number LS-C26224. It has been validated for use in the following assays: IHC-P.
Antibody Target:	RAD51 Homolog (RAD51)
Synonyms:	RAD51, BRCC5, HRAD51, HsRad51, HsT16930, RAD51A, RAD51D, RAD51L3, RECA, Rhp51
Host:	RAD51 antibody was produced in Mouse
Antigen Species:	RAD51 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Recombinant protein
Immunogen:	RAD51 antibody was raised against recombinant Rad51 protein. Cellular Localization: Nuclear. MW of Antigen: 37-43kD
Antibody Specificity:	Recognizes human Rad51. Species cross-reactivity: mouse and rat.
Predicted Species Reactivity:	Mouse, Rat
Purification:	Protein G Column
Presentation:	10 mM PBS, pH 7.4.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2597 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2597 was determined to be 10 ug/ml. Immunoprecipitation (Native verified): 2 ug/mg protein lysate. Use Protein G. Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: HeLa cells and testis.
Antibody Uses:	Immunohistochemistry (IHC) (2 - 4 µg/ml), Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	1 mg/ml