Rabbit polyclonal to CD44 (aa728-742) / Phagocytic glycoprotein 1 / PGP-1: Affinity purified IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

CDw44,Epican,Extracellular matrix receptor III,ECMR-III,GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Heparan sulfate proteoglycan,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD_antigen: CD44

 

Antibody_Isotype
(Antibody only) : IgG

 

Application_Details :

WB: Western blot: reduced RIPA samples or membrane preparations (0.1-.5 ug/mL) dependent upon antigen load and antibody incubation times and detection substrate used.

IH: frozen or acetone fixed tissue: 0.5-1.0 ug.mL, permeabilization is necessary as epitope is internal
IH(P): 1-2 ug/mL with HEIR treatment required.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1519

 

Description (Long Description) : 

The protein identified as CD44 is also known as the receptor for hyaluronic acid (HA). The protein mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Biosensis' anti-CD44 rabbit polyclonal antibody is a new antibody made against a synthetic peptide epitope near the very C-terminus, cytoplasmic domain of human CD44. The antibody has been shown to react with multiple species including human, mouse and rat, in both western blot and immunohistochemistry applications including formalin fixed, paraffin embedded tissues. See applications detail for specific use instructions.

 

Expiry_Date : 

12 months after purchase.

 

Format (Form) : 

lyophilized.

 

Immunogen (Antigen) :A synthetic peptide corresponding to a sequence at the C-terminus of human CD44 cytoplasmic domain aa 728-742. The immunogen differs by only single amino acid between human, mouse and rat proteins.

 

Kit_Components :

 

MSDS (File name) :