Rabbit polyclonal antibody to rh c-FOS: Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS

 

Antibody_Isotype
(Antibody only) : IgG

 

Application_Details :

Immunohistochemistry (IHC) and immunocytochemistry (ICC): 0.2-1.0 µg/mL. This antibody has been shown to work on 4% PFA fixed mouse brain sections.

Western blotting (WB): 0.5-1.0 µg/mL. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1577

 

Description (Long Description) : 

FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).

 

Expiry_Date : 

12 months after purchase if unopened and stored as indicated.

 

Format (Form) : 

Lyophilized from a solution containing 3% trehalose in PBS, pH 7.4, without preservatives.

 

Immunogen (Antigen) :Full length, E.coli-derived recombinant human c-FOS protein.

 

Kit_Components :

 

MSDS (File name) :