Rabbit polyclonal antibody to Hypoxia-inducible factor-1 alpha (8-21): Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; HIF1A; MOP1;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blotting (WB), IHC-P. A concentration of 0.1-0.5 µg/mL is recommended for WB. Human HIF-1 alpha (isoform 1) has a predicted length of 826 residues and a MW of 93 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect HIF1A in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required by boiling the formalin/paraffin sections in 10mM citrate buffer, pH6.0 for 20 mins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

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Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1520

 

Description (Long Description) : 

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Uniprot UniProtKB - Q16665 (HIF1A_HUMAN).

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3

 

Immunogen (Antigen) :A synthetic peptide corresponding to a sequence at the N-terminus of human HIF-1-alpha (8-21aa NDKKKISSERRKEK), different from the related rat and mouse sequences by two amino acids.

 

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