Rabbit polyclonal antibody to human Sonic Hedgehog protein (34-50): Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

SHH; HHG-1;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Sonic Hedgehog protein (precursor) has a predicted length of 462 amino acids and MW of 50 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=552

 

Description (Long Description) : 

Sonic Hedgehog protein (SHH) is essential for a variety of patterning events during embryo development. SHH is expressed in fetal intestine, liver, lung, and kidney. The SHH precursor protein is autocatalytically cleaved into two products. The C-terminal portion is involved in the proteolysis of the precursor and cholesterol transferase activity. The N-terminal portion contains signalling activity.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3

 

Immunogen (Antigen) :A synthetic peptide (RHPKKLTPLAYKQFIPN) corresponding to a region (34-50) from human Sonic Hedgehog protein (SHH). To enhance the immunological response, this peptide was coupled to carrier protein BSA.

 

Kit_Components :

 

MSDS (File name) :