Rabbit polyclonal antibody to human Smad 2/3: Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Mad3; SMAD 3; Mothers against DPP homolog 3; Mothers against DPP homolog 2; MAD-2; SMAD 2; JV18-1; MADH2; MADH3;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Smad2 is 467 amino acids and MW of 52 kDa. The predicted length of Smad3 is 425 amino acids and MW of 48 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Smad 2/3 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Heat mediated antigen retrieval is required. A concentration of 0.4-1.0 µg/mL is recommended for IHC-F. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=480

 

Description (Long Description) : 

Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription. Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD). The R-SMAD class includes Smad2 and Smad3.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Liquid (0.5mL). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4

 

Immunogen (Antigen) :A synthetic peptide (EQNGQEEKWCEKAVKS) corresponding to a region from the N-terminus of human Smad2 and Smad3.

 

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MSDS (File name) :