Rabbit polyclonal antibody to human Matrix Metalloproteinase 2 (MMP2, 475-490): Affinity purified
Antibodies
- 貨號 Cat. No.
- R-1381-100
- 規格 Size
- 100 µg
- 品牌 Brand
- Biosensis
- Application 應用
- IH,Frozen, IH(P), Paraffin embedded 、 WB
- Reactivity
- Chicken (Predicted) 、 Cow (Predicted) 、 Horse (Predicted) 、 Human 、 Mouse (Predicted) 、 Pig (Predicted) 、 Rat (Predicted)
- Host
- Rabbit
- Clonality
- Polyclonal
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
72 kDa gelatinase; Gelatinase A; MMP-2; TBE-1
Antibody_Isotype
(Antibody only) : IgG
Application_Details :
Western Blotting (WB): 1:5,000 - 1:10,000. MMP2 appears as two bands at apparent molecular weights of 66 and 72 kDa. Immunohistochemistry (IHC): 1:500-1:1,000.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1579
Description (Long Description) :
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. MMP2 isoform 2 mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. Catalytic activity of MMP2 causes cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. (Ref: uniprot.org).
Expiry_Date :
12 months after purchase (unopened vial).
Format (Form) :
Lyophilized from PBS pH 7.4, containing 3% trehalose, without preservatives.
Immunogen (Antigen) :Human C-terminal peptide (amino acids 475-490).
Kit_Components :
MSDS (File name) :
