Rabbit polyclonal antibody to human Insulin receptor subunit alpha (59-75): Affinity purified

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

IR; EC 2.7.10.1; CD220; INSR;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Insulin receptor (isoform Long) has a predicted length of 1382 amino acids and MW of 156 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=595

 

Description (Long Description) : 

The Insulin receptor binds insulin and has a tyrosine-protein kinase activity. The receptor is a tetramer of 2 alpha and 2 beta subunits linked by disulfide bonds. The alpha subunit (135 kDa) is extracellular and contributes to the formation of the ligand-binding domain whereas the beta subunit (95 kDa) contains the kinase domain and a transmembrane domain. At least two isoforms of the Insulin receptor are produced by alternative splicing.

 

Expiry_Date : 

12 months after purchase unopened

 

Format (Form) : 

Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3

 

Immunogen (Antigen) :A synthetic peptide (HLQILLMFKTRPEDFRD) corresponding to a region (59-75) from human Insulin receptor (isoform Long). To enhance the immunological response, this peptide was coupled to carrier protein BSA.

 

Kit_Components :

 

MSDS (File name) :