Rabbit polyclonal antibody to human GAD67 (14-122): Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

GAD; GAD-67; GAD1; Glutamate decarboxylase 1;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The detection limit for GAD67 is approximately 0.25ng/lane under reducing conditions. Human GAD67 has a predicted length of 594 residues and MW of 67 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.Not yet tested in other applications.

 

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Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1535

 

Description (Long Description) : 

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) and several isoforms of GAD67 and GAD65 have been discovered. Clustal alignments show 64.1% amino acid identity between GAD65 and GAD67 across the entire protein with higher identity being toward the C-terminal ends of the proteins. Expression of the two proteins and of the specific isoforms (for instance isoform 3 of GAD67 is not expressed in brain) also varies. This antibody reacts with GAD67 and has not yet been tested directly on recombinant GAD65 for cross reactivity. Blots of whole brain extracts appear as a single band at 67 kDa. The sequence used as an immunogen does have some identity with GAD65 however it is slight and broken up by multiple gaps.

 

Expiry_Date : 

12 months after purchase.

 

Format (Form) : 

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

 

Immunogen (Antigen) :E.coli-derived human GAD67 recombinant protein (14-122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

 

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