Rabbit monoclonal antibody to human FGF Receptor 2

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

FGFR-2; EC 2.7.10.1; Keratinocyte growth factor receptor 2; CD332; FGFR2; BEK; KGFR; KSAM;

 

Antibody_Isotype
(Antibody only) : IgG

 

Application_Details :

Western Blotting (WB). A dilution of 1:500-1:2,000 is recommended for WB. R-1776-100 has an epitope located in the extracellular domain portion of FGFR2. In tests using C6 cells, this antibody detects a 135kDa band under reducing conditions Immunoprecipitation (IP). A concentration of 1:50 is recommended for IP in a lysis buffer that contains no more than 0.5% detergent as well as proteinase inhibitors. Detection can be via R-1776-100 in westerns following, IP enrichment however rabbit heavy chain (50-55~kDa) and light chains (~23-25kDa) will also be detected by the anti-rabbit used to detect R-1776-100 on westerns unless biotinylation of R-1776-100 is used. As always, Biosensis recommends optimal dilutions/concentrations should be determined by the end user for their specific experimental design.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1595

 

Description (Long Description) : 

FGF receptor 2 (FGFR2) is a member of the fibroblast growth factor receptor family and is Single-pass type I membrane protein and a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. At least 20 isoforms are produced by alternative splicing. Because of its many isoforms, FGF2 receptor can demonstrate a wide range of sizes on reduced westerns generally ranging from ~135kDa to 50 kDa in size.  FGFR2 is highly expressed in developing human tissues and localizes to plasma, Golgi, and cytoplasmic vesicles. After ligand binding, the activated receptor is rapidly internalized and degraded.

 

Expiry_Date : 

12 months after date of receipt unopened.

 

Format (Form) : 

Liquid in PBS pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

 

Immunogen (Antigen) :A synthetic peptide from human FGF receptor 2.

 

Kit_Components :

 

MSDS (File name) :