Rabbit antibody to ATG5: IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP

 

Antibody_Isotype
(Antibody only) : IgG

 

Application_Details :

Flow Cytometry (2 ug/10^6 cells), IHC, immunofluorescence, WB. Use a dilution of 1:200 to 1:1000 for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=210

 

Description (Long Description) : 

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.

 

Expiry_Date : 

Six months after purchase

 

Format (Form) : 

Lyophilized

 

Immunogen (Antigen) :A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

 

Kit_Components :

 

MSDS (File name) :