Rabbit anti human PAI-1 affinity purified high titer IgG fraction

Product: Rabbit anti human PAI-1 IgG fraction, affinity purified high titer – ASHPAI-GF-HT

Date: July 2003 Lot: Sample

Molecular Weight: ~ 160,000 by SDS PAGE

Physical Specifications:

Physical Appearance: colorless frozen solution

Solubility: < 10 mg/mL

Spectrophotometric Data:

Ultraviolet Absorbance (280nm) = 0.82 0.1% = 1.36

Concentration = 0.6 mg/mL

Application:

This preparation of polyclonal antibody has been used successfully in western blots and ELISA based assays.

Use at ~ 1:100,000 for ELISA.

Use at ~ 1:10,000 for Western Blots.

Considerations:

Storage Conditions: Store frozen at -70ï‚°C – stability > 1yr

Buffer Composition: 0.05M Sodium Phosphate; 0.1 M NaCl;

1 mM EDTA; pH 6.6