rAa1
- 貨號 Cat. No.
- RTA-400
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
rAa1
A Novel Blocker of Shaker-B and IA K+ Channels.
| Product#: | RTA-400 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
| M.W.: | 3868 |
| Sequence: | QNETN KKCQG GSCAS VCRRV IGVAA GKCIN GRCVC YP |
| Purity: | >98% |
| Bioassay: |
| The biological activity of rAa1 was tested using two-electrode voltage clamp recording from Xenopus oocytes expressing the Shaker B channels. |
 |
rAa1, inhibition of Shaker B channels expressed in Xenopus oocytes. Left: Current responses to 100 ms depolarization to 0 mV from holding potential of ?FONT mV, delivered every 10 seconds before (red) and during (green) application of about 1 m Right: Time course of amplitude inhibition. The horizontal bars indicate the periods of Aa1perfusion (with increasing concentrations). M rAa1. |
| References: |
1. | Pisciotta, M. et al. (1998) Biochem. Biophys. Res. Comm. 242, 287. |
2. | Pisciotta, M. et al. (1998) Eur. Biophys. J. 27, 69. |
3. | Pisciotta, M. et al. (2000) Biochim. Biophys. Acta. 1468, 203. |
Native Aa1 was originally isolated from Androctonus australis scorpion venom.
rAa1 is a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.
Native Aa1 blocks Shaker B channels expressed in Xenopus oocytes with IC50=4.5 然.1 In addition
the Aa1 toxin was shown to block the fast (A-type) K+ current in cerebellum granular cells
(IC 50 =134 nM, 2 or Kd = 150 nM3). In both cases the block is non voltage dependent and
does not affect kinetic parameters of the K+ currents.
rAa1 is a recombinant peptide expressed in and extracted from E. coli and purified to homogeneity.
Native Aa1 blocks Shaker B channels expressed in Xenopus oocytes with IC50=4.5 然.1 In addition
the Aa1 toxin was shown to block the fast (A-type) K+ current in cerebellum granular cells
(IC 50 =134 nM, 2 or Kd = 150 nM3). In both cases the block is non voltage dependent and
does not affect kinetic parameters of the K+ currents.