QX-314 (Cl- salt)
- 貨號 Cat. No.
- Q-150
- 規格 Size
- 500 mg 1g
Description
QX-314 (Cl- salt)
A Local Anesthetic Blocker of Nav Channels
| Product#: | Q-150 |
| Sizes: |
| 500 mg |
| 1 g |
This local anesthetic is a blocker of voltage-dependent Na+ channels. QX-314 blocks all Nav channels once exposed to the intracellular face of the channel protein. However, this compound can penetrate the cell interior once applied extracellulary, via certain types of Nav channels, it can permeate and exert blocking activity via TTX sensitive channels.1 Intracellular recording from inferior olivary neurons in brain stem slice preparation demonstrated that Na+ - dependent action potential was completely blocked for approximately five minutes after impalement with a microelectrode containing 50mM QX-314, whereas the low-threshold Ca2+ spike was still generated.2 QX-314 is widely used to block Nav channels as well as a tool in neurophysiological recordings.2-5
| M.W.: | 289.85 |
 |
| Purity: | >99% |
| Bioassay: |
| Inhibition of Nav1.5 channel currents by increasing concentrations of QX-314. |
 |
Dose response of QX-314 on inward currents in Xenopus oocytes injected with Nav1.5 RNA. Using two electrode voltage clamps, oocyte membrane potential was held at -100 mV and currents were elicited by 25 ms depolarization to -20 mV, delivered every 5 seconds. Left: Dose response curve for one experiment. Right: Traces before (red) and during bath application of different (indicated with arrow) QX-314 concentrations. |
| References: |
1. | Sunami, A. et al. (2000) P.N.A.S. USA 97, 2326. |
2. | Llinas, R. and Yarom, Y. (1981) J. Physiol. 315, 549. |
3. | Congar, P. et al. (1995) J. Neurophysiol. 73, 421. |
4. | Li, D.P. et al. (2005) J. Neurophysiol. 93, 393. |
5. | Koike-Tani, M. (2005) J. Neurosci. 25, 199. |