Proteins and Peptides
PTK2 / FAK Antibody (LS-B2522)
- 貨號 Cat. No.
- LS-B2522
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2522 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C15121. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | PTK2 / FAK |
| Synonyms: | PTK2 Antibody, FADK Antibody, fadk1 Antibody, FAK Antibody, FAK1 Antibody, focal adhesion kinase Antibody, focal adhesion kinase 1 Antibody, FRNK Antibody, p125FAK Antibody, pp125 FA kinase Antibody, pp125fak Antibody, protein tyrosine kinase 2 Antibody, ptk2 Antibody |
| Family: | Protein Kinase |
| Subfamily: | Focal Adhesion Kinase |
| Host: | PTK2 antibody was produced in Rabbit |
| Antigen Species: | PTK2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | PTK2 antibody was raised against synthetic phosphopeptide derived from the region of human FAK that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog. |
| Antibody Specificity: | Reactivity: Human FAK. Mouse, rat, chicken and frog (100% homologous) FAK have not been tested, but are expected to react. Some cross-reactivity with Pyk2 may still be experienced in cases where Pyk2 is overexpressed relative to FAK within the same cell system. |
| Antigen Modification: | pTyr576 |
| Predicted Species Reactivity: | Chicken, Frog, Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide, 50% glycerol. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2522 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2522 was determined to be 1:100. Positive control: Chicken embryo fibroblasts (CEFs) expressing FAK protein and plated on fibronectin and treated with vanadate. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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