Proteins and Peptides
PTK2 Antibody (LS-B2153)
- 貨號 Cat. No.
- LS-B2153
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2153 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C15116. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Focal Adhesion Kinase 1 (PTK2, FAK1) |
| Synonyms: | PTK2, FADK, fadk1, FAK, FAK1, focal adhesion kinase, focal adhesion kinase 1, p125FAK, pp125 FA kinase, pp125fak, protein tyrosine kinase 2, ptk2 |
| Family: | Protein Kinase |
| Subfamily: | Focal Adhesion Kinase |
| Host: | PTK2 antibody was produced in Rabbit |
| Antigen Species: | PTK2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | PTK2 antibody was raised against synthetic phosphopeptide derived from the region of human FAK that contains tyrosine 407. The sequence is conserved in mouse, rat and chicken. |
| Antibody Specificity: | Reactivity: Human FAK. Mouse, rat, chicken (100% homologous) and frog (92%) FAK have not been tested, but are expected to react. |
| Antigen Modification: | pTyr407 |
| Predicted Species Reactivity: | Chicken, Frog, Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | DPBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol. |
| Recommended Storage: | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2153 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2153 was determined to be 1:200. Positive control: Chicken embryo fibroblast (CEF) cells expressing FAK protein and plated on fibronectin. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:200), Immunocytochemistry (ICC), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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