Proteinase Inhibitor 9 (PI9) Antibody

Antibody Target :Proteinase Inhibitor 9 (PI9)

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Recombinant human full length PI9

Accession#: P50453

Gene ID: 5272

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) mouse monoclonal antibody from culture supernatant diluted in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu

Application: WB

Application & Usage: Western blotting (2-4 µg/ml) and immunohistochemistry (20 µg/ml). It recognizes 42 kDa native human PI-9 and the 63 kDa PI-9 complexed to granzyme B. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Proteinase inhibitor 9 (PI-9, also designated cytoplasmic antiproteinase 3, or CAP3) is a 42 kDa member of the ovalbumin family of serpins that is expressed in placenta, lung and cytotoxic lymphocytes. PI-9 is a potent inhibitor of granzyme B and of granzyme B-mediated apoptosis, and is also an inhibitor of capase-1 and, to a smaller extent, caspase-4 and caspase-8. PI-9 expression is also upregulated in response to inflammatory stimuli. This upregulation protects cells from apoptosis induced by endogenously expressed or released granzyme B, particulary during target cell killling.